5ul of protein ladder samples was loaded into each lane. *NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. ► Enhanced transfer, wash, and stripping durabilities.Īpproximately 0.2~0.4 mg/ml of each protein in buffer ( 62.5 mM Tris-H3PO4 (pH 7.5 at 25 ☌), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.)ĥ μl of BLUltra Prestained Protein Ladder (PM001-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to the nitrocellulose membrane. ► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system. This technique should prove valu- able for immunodetection of a molecu- lar weight reference ladder in Western blot analysis of proteins using chemilu. ► Better performance for higher molecular weight proteins. ► Higher Added Value: Tri-colors and 10 bands with premium quality. ► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa. That's why we're pleased to launch the color-enhanced BLUltra prestained protein standards.Īs you may see the comparison data shown below, BLUltra shows a better resolution than the BLUeye & BlueRAY for the protein bands from 95 kDa to 270 kDa. Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. Caution: Not intended for human or animal diagnostic or therapeutic uses. * supplement data should be considered for more accurate adjustment. Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Prestained Protein Marker with increased brightness provides robust visualization for western blotting workflow, identifying target proteins on the gel or. Apply more for thicker (>1.5 mm) or larger gel.2~3 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Migration patterns of BLUltra Prestained Protein Ladder in different electrophoresis conditions are listed below : Guide for Molecular Weight Estimation (kDa) The ladder is supplied in the gel loading buffer and is ready to use. The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |